Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of F?rster resonance energy transfer

Datinská Vladimíra; Klepárník Karel; Bel?ánová Barbora; Minárik Marek; Foret Franti?ek

摘要

Abstract >The synthesis and determination of the structure of a F?rster resonance energy transfer probe intended for the detection of specific nucleic acid sequences are described here. The probe is based on the hybridization of oligonucleotide modified quantum dots with a fluorescently labeled nucleic acid sample resulting in changes of the fluorescence emission due to the energy transfer effect. The stoichiometry distribution of oligonucleotides conjugated to quantum dots was determined by capillary electrophoresis separation. The results indicate that one to four molecules of oligonucleotide are conjugated to the surface of a single nanoparticle. This conclusion is confirmed by the course of the dependence of F?rster resonance energy transfer efficiency on the concentration of fluorescently labeled complementary single‐stranded nucleic acid, showing saturation. While the energy transfer efficiency of the probe hybridized with complementary nucleic acid strands was 30%, negligible efficiency was observed with a noncomplementary strand. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >用于f？rste共振能量转移探针的结构的合成和确定这里描述了特定核酸序列的检测。探针基于寡核苷酸改性量子点的杂交，具有荧光标记的核酸样品，导致由于能量转移效应引起的荧光发射的变化。通过毛细管电泳分离测定与量子点缀合的寡核苷酸的化学计量分布。结果表明，一到四个寡核苷酸分子与单个纳米颗粒的表面缀合。该结论是通过F？脊柱螺旋共振能量转移效率对荧光标记的互补单链核酸浓度的依赖性来确认的结论，显示出饱和度。虽然用互补核酸链杂交的探针的能量转移效率为30％，但用非互相股线观察到可忽略的效率。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2018年第14期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Datinská Vladimíra&option=202" target="_blank" rel="nofollow">Datinská Vladimíra;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Klepárník Karel&option=202" target="_blank" rel="nofollow">Klepárník Karel;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bel?ánová Barbora&option=202" target="_blank" rel="nofollow">Bel?ánová Barbora;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Minárik Marek&option=202" target="_blank" rel="nofollow">Minárik Marek;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Foret Franti?ek&option=202" target="_blank" rel="nofollow">Foret Franti?ek;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Institute of Analytical Chemistry of the CAS v. v. i.Brno Czech Republic;</p> <p>Institute of Analytical Chemistry of the CAS v. v. i.Brno Czech Republic;</p> <p>Center for Applied Genomics of Solid Tumors (CEGES)Genomac Research InstitutePrague Czech Republic;</p> <p>Center for Applied Genomics of Solid Tumors (CEGES)Genomac Research InstitutePrague Czech Republic;</p> <p>Institute of Analytical Chemistry of the CAS v. v. i.Brno Czech Republic;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=capillary electrophoresis&option=203" rel="nofollow">capillary electrophoresis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=F?rster resonance energy transfer&option=203" rel="nofollow">F?rster resonance energy transfer;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=nucleic acids&option=203" rel="nofollow">nucleic acids;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=quantum dots&option=203" rel="nofollow">quantum dots;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=sensors&option=203" rel="nofollow">sensors;</a> </p> <div class="translation"> 机译：毛细管电泳;F？脊柱阵线共振能量转移;核酸;量子点;传感器; 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2019</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120111176408.html">基于核酸适配体-量子点结合苏云金芽孢杆菌孢子的荧光测定法</a> <b>[P]</b> . <span> 中国专利： CN108663343A </span> <span> . 2018-10-16</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/061204937261.html">量子点表面配体含量的测定方法与量子点墨水配制方法</a> <b>[P]</b> . <span> 中国专利： CN109932358B </span> <span> . 2022.03.01</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130409254760.html">COVALENT CONJUGATES OF NUCLEIC ACIDS WITH 1,4-BIS-PHENYLAZO-BENZENE AND PHENYLAZO-PHENAZINE DERIVATIVES AS DARK QUENCHERS OF DONOR ACCEPTOR ENERGY TRANSFER FOR USE E.G. IN AUTOMATED NUCLEIC ACID SYNTHESIS</a> <b>[P]</b> . <span> 外国专利：  PT2333113T </span> <span> . 2017-09-08</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：核酸与1,4-双-苯并-苯并苯衍生物和苯并-苯并-苯并菲衍生物的共价共轭物，作为配体接受者能量转移的暗态猝灭剂，例如在自动核酸合成中 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130409412753.html">Covalent conjugates of nucleic acids with 1,4-bis-phenylazo-benzene and phenylazo-phenazine derivatives as dark quenchers for donor-acceptor energy transfer for use e.g. in an automated nucleic acid synthesis</a> <b>[P]</b> . <span> 外国专利：  DK2333113T3 </span> <span> . 2017-09-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：核酸与1,4-双-苯基偶氮苯和苯基偶氮-吩嗪衍生物作为暗淬灭剂的共价缀合物，用于例如供体-受体能量转移在自动核酸合成中 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130411065104.html">Covalent conjugates of nucleic acids with 1,4-bis-phenylazo-benzene and phenylazo-phenazine derivatives as dark quenchers of donor acceptor energy transfer for use e.g. in automated nucleic acid synthesis</a> <b>[P]</b> . <span> 外国专利：  EP2333113B1 </span> <span> . 2017-08-16</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：核酸与作为供体受体能量转移的黑暗猝灭剂的1,4-双-苯基偶氮苯和苯基偶氮吩嗪衍生物的共价缀合物，例如用于在自动核酸合成中 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" 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