oligonucleotide microarray for simultaneous detection of two mutations causing the disease of charcomarie - tooth in north africa.

摘要

the present invention relates to a dna chip for the simultaneous analysis of mutations responsible for the disease, charcot marie tooth by means of multiplex pcrs with the primer extensionthe specific oligonucleotide probes were designed and printed 19 mutations on different blades of aldehydes using spotters) without contact.the allelic discrimination is based on the extension of the probe which is complementary to the pcr products.the original chip allows the rapid diagnosis, precise and expensive 19 mutations responsible for charcot marie tooth in 8 patients, at the same time.and can, therefore, be useful in the guidance of clinical follow-up and genetic counselling.