Method for purifying human serum albumin from transgenic rice grain

摘要

A method for isolating and purifying recombinant human serum albumin from transgenic rice grain, sequentially comprising the steps of: 1) subjecting recombinant human serum albumin crude extract to cation exchange chromatography to obtain primary product I; 2) subject the primary product I to anion exchange chromatography to obtain the secondary product II; 3) subject the secondary product II to hydrophobic chromatography to obtain purified recombinant human serum albumin; wherein the method further comprises a step of subjecting the secondary product II to ceramic hydroxyapatite chromatography before the hydrophobic chromatography of step 3).