首页> 外国专利> Kit and method to detect and quantify the natural variants of the hepatitis C virus, genotype 1, subtype a), which comprise the Q80K mutation.

Kit and method to detect and quantify the natural variants of the hepatitis C virus, genotype 1, subtype a), which comprise the Q80K mutation.

机译:用于检测和定量包含Q80K突变的丙型肝炎病毒的自然变异的试剂盒和方法,基因型1,亚型。

摘要

The invention relates to a diagnostic kit or device, useful for use in pyrosequencing tests, which optionally comprise the reagents necessary to carry out a retrotranscription and amplification process via the chain reaction of the polymerase (RT-PCR) comprising at least one/a number of primer(s) suitable for being hybridised with the SEQ ID NO: 1 and permitting the amplification of a fragment of a maximum of 400 RNA base pairs of the hepatitis C virus, including the natural resistance variant "Q80K", in an RT-PCR method for the purpose of detecting and quantifying the natural variants of the hepatitis C virus, genotype 1, subtype a, comprising the Q80K mutation.
机译:诊断试剂盒或装置技术领域本发明涉及诊断试剂盒或装置,其可用于焦磷酸测序测试,其任选地包含通过包含至少一个/多个数目的聚合酶(RT-PCR)的链反应进行逆转录和扩增过程所必需的试剂。适用于与SEQ ID NO:1杂交的引物的片段,并允许在RT-PCR中扩增最大400丙型肝炎病毒RNA碱基对的片段,包括天然抗性变体“ Q80K”用于检测和定量包含Q80K突变的丙型肝炎病毒基因型1,亚型a的自然变异的PCR方法。

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