Use of triple structure lentivirus sequences for the nuclear import of nucleotide sequences

摘要

Use or of a polynucleotide for the manufacture of a recombinant plasmid vector intended for the production, by cotransfection of transcomplementation plasmids, of recombinant lentiviral particles devoid of lentiviral genes and intended for transduction to eukaryotic cells of a target nucleotide sequence, particles where said polynucleotide is the determinant of nuclear import, said polynucleotide being derived from a lentivirus and consisting of an active region in central initiation cis (cPPT) containing at least 10 nucleotides, of an active region in termination cis (CTS) containing at least 10 nucleotides, and an internal concatenation of nucleotides flanked by two regions, said CTS region and said internal concatenation of nucleotides a sequence having a size of 80 to 120 nucleotides, said nucleotide being capable of forming a triplet structure during the ret genome rotranscription of said particles in the target eukaryotic cell, said plasmid being devoid of lentiviral genes and comprising a recombinant nucleotide sequence comprising - said nucleotide sequence of interest placed under the control of transcriptional and expression regulatory signals , - back-transcription, expression and encapsulation regulatory signals derived from a lentivirus and - said polynucleotide, whose cis active regions cited are inserted in a functional orientation with said regulatory signals derived from a lentivirus.