Treatment of degeneration of intervertebral discs using cells derived from human umbilical cord tissue

摘要

Cells obtained from human umbilical cord tissue, in which the umbilical cord tissue is substantially free of blood, where the cells: can be obtained by enzymatic digestion of human umbilical cord tissue that is substantially free of blood with a metalloprotease, a neutral protease and a mucolytic protease; they are capable of self-renewal and expansion in cultivation; they have the potential to differentiate; and have the following characteristics: a) express CD10, CD13, CD44, CD73, CD90, PDGFR-alpha, PD-L2, and HLA-A, B, C as detected by flow cytometry; b) not express CD31, CD34, CD45, CD80, CD86, CD141, CD178, HLA-G, CD117 and HLA-DR, DP, DQ as detected by flow cytometry; c) not express telomerase; and d) express, in relation to a human fibroblast, mesenchymal stem cells, or iliac crest of bone marrow cells, increased levels of interleukin 8, reticulon 1, chemokines (CXC motif) ligand 1 or chemokines (CXC motif) ligand 3 , for use in the treatment of a disease or condition related to degeneration of the intervertebral disc by administering the cells in the nucleus pulposus or fibrous ring of the intervertebral disc