15 variants lipoprotein (LP15) of Vibrio cholerae as an additive anti-interference TpN17 based immunoassays for the detection of anti-Treponema

摘要

A method for detecting antibodies TpN17 antigen Treponema pallidum in an isolated sample, in which a peptide sequence lipoprotein 15 Vibrio cholerae (VcLp15) as a reagent used to reduce interference and to minimize false positive results, wherein said sequence comprises VcLp15 amino acid residues 26-163 of SEQ ID NO. 1 or 2 and in which they may be truncated from 1 to 5 amino acids from the N-terminal or C-terminal or both ends of said sequence VcLp15 and wherein said sequence Vc LP15 can be modified by conservative amino acid substitutions so the three dimensional structure of lipoprotein 15 Vibrio cholerae (VcLp15) not suffer changes.