BIOTECHNOLOGICAL BREEDING METHOD FOR OBTAINING ANTIVIRAL SEEDLESS GRAPES

摘要

Disclosed is a biotechnological breeding method for obtaining antiviral seedless grapes. The method comprises the following steps: obtaining zygotic embryos containing seedless genes by carrying out hybridization between varieties of seedless grapes and embryo rescue, and inducing grape somatic embryogenesis by means of the zygotic embryos; and carrying out agrobacterium tumefaciens-mediated genetic transformation by using RNAi antiviral vectors containing conservative gene segments of grape virus coat protein (CP), so that a grape material which is both antiviral and seedless can be obtained from transformed regeneration plants.