Assay methods for detecting analytes in samples involving the use of nanoparticles that comprise a signal inducing agent, e.g., a transition- metal catalyst. After binding to an analyte, the nanoparticle is dissociated by a trigger to release the signal inducing agent, which releases a detectable signal via a physical or chemical reaction. Also described herein are nanoparticles, and compositions and uses thereof, where the nanoparticle comprises chemiluminophore, a chemiluminophore precursor, a soluble absorber, or a soluble absorber precursor, that effects chemical reactions that serve as signal amplifiers.
展开▼
