Disclosed is a circulating tumor cell identification kit, comprising a capture probe, an amplification probe, and a labeled probe for each marker gene mRNA. The marker genes comprise the following two types: at least two epithelial cell marker genes mRNA selected from the group consisting of EPCAM, E-cadherin, CEA, KRT5, KRT7, KRT17, and KRT20, and at least two mesenchymal cell marker genes mRNA selected from the group consisting of VIMENTIN, N-cadherin, TWIST1, AKT2, ZEB2, ZEB1, FOXC1, FOXC2, SNAI1, and SNAI2.
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