METHOD FOR PREPARING RECOMBINANT PHYCOBILIPROTEIN CONCATEMER HAVING HIGH FLUORESCENCE INTENSITY

摘要

Provided is a method for preparing a recombinant phycobiliprotein concatemer having high fluorescence intensity. A streptavidin gene is connected to an allophycocyanin α-subunit gene, and then one or more allophycocyanin α-subunit genes are connected. The obtained fusion gene is co-expressed with a phycobiliprotein lyase gene and a phycoerythrobilin biosynthetic enzyme gene in Escherichia coli, so as to obtain a recombinant phycobiliprotein concatemer having biotin binding ability and high fluorescence intensity, which can be used as a marker for immunofluorescence detection.