METHOD FOR ESTIMATING ALLELE-SPECIFIC GENE EXPRESSION FREQUENCY, COMPUTER SYSTEM USED FOR ESTIMATION, AND PROGRAM USED FOR ESTIMATION

摘要

The present invention addresses the problem of providing a statistical probabilistic means capable of accurately estimating an allele-specific gene expression in various organisms. The inventors have discovered that this problem can be solved by using a computer to perform steps (1) - (4) with respect to data wherein read information for cDNA of a polyploid organism.(1) Digitalization of each isoform in each read and an expected mapping number with respect to each allele of the isoform.(2) Calculation of a total number of expected mappings by means of the total for each isoform in the expected mapping number digitized in (1), and calculation of the portion of each allele in the total number of expected mappings.(3) Calculation of (i) an isoform quantitative ratio and (ii) an allele-specific selection related to the total number of expected mappings calculated in (2).(4) Certification of a convergence value obtained by a loop process for the aforementioned steps as the optimal data for the allele-specific gene expression.