GENETIC MARKER FOR DETECTING CAUSATIVE VIRUS OF RED SEA BREAM IRIDOVIRAL DISEASE, AND CAUSATIVE VIRUS DETECTION METHOD USING SAME

摘要

The present invention relates to a genetic marker for discriminating and detecting a causative virus of a marine creature infectious disease, i.e., the causative virus of red sea bream iridoviral disease, and a causative virus discrimination and detection method using same and, more specifically, to a method comprising: selecting and amplifying a DNA base sequence for coding (I) a particular gene of red sea bream iridovirus (RSIV)/infectious spleen and kidney necrosis virus (ISKNV) or (ii) a particular gene which discriminates RSIV from RSIV/RSKNV; then hybridizing a peptide nucleic acid (PNA) which specifically recognizes the amplified product; obtaining a temperature-based melting curve by controlling the temperature of the hybridized product; and determining a virus type from a melting temperature through the analysis of the obtained melting curve, or detecting the status of infection of fishes with the virus type.