AR SRM/MRM SRM/MRM ASSAY FOR THE ANDROGEN RECEPTOR AR PROTEIN

摘要

Current disclosures are particularly advantageous for quantifying AR proteins directly in biological samples immobilized in formalin by means of selective reaction monitoring (SRM) mass spectrometry, or by methods of what may also be referred to as multiple reaction monitoring (MRM) mass spectrometry From the receptor protein (AR), certain peptides, and the ionization characteristics of the peptides, are provided. Such a biological sample is chemically preserved and fixed, wherein the biological sample is immobilized on a formalin-fixed tissue / cell, a formalin-fixed / paraffin embedded tissue (FFPE) tissue / cell, a FFPE tissue block, / RTI and / or tissue cultured cells embedded in paraffin, with formaldehyde-treated tissues and cells containing agonist / fixative. Protein samples are prepared from the biological samples using liquid tissue reagents and protocols, and the AR proteins are quantified in liquid tissue samples by the method of SRM / MRM mass spectrometry by quantifying at least one of the described peptides in a protein sample. If these peptides are present in a modified or unmodified form, these peptides can be quantified. An example of a modified form of the AR peptide is phosphorylation of the tyrosine, threonine, serine, and / or other amino acid residues in the peptide sequence.