Identifying Method Of Species Or Origin About Fish And Shellfish Using Electrochemical Detecting Signal

摘要

The present invention relates to a gene reading method for comparing and analyzing an electrochemical detection signal for discrimination of species and origin of fish and shellfish. More particularly, the present invention relates to a gene reading method for amplifying a target gene, The present invention relates to a gene reading method capable of distinguishing species and origin of fish by comparing electrochemical signal differences before and after hybridization using an electrochemical method, followed by hybridization of probe genes. Accordingly, the present invention provides a method for identifying species and origin of fish and shellfish using an electrochemical detection signal, comprising the steps of: i) amplifying target DNA of fish and shellfish to be known about species and origin in a gene amplification tube using polymerase chain reaction (PCR) ; Ii) immobilizing the probe DNA with known species and origin information of the fish and shellfish on the gene-reading chip; Iii) measuring an electrochemical signal on the electrode of the gene reading chip on which the probe DNA is mounted; (Iv) extracting the target DNA from the gene amplification tube, and hybridizing the DNA with the probe DNA; V) measuring an electrochemical signal on the electrode of the gene reading chip on which the probe DNA and the target DNA are mounted; Vi) comparing and reading the measured electrochemical signals; (Fe (CN) ₆ ] ^3- ) is used as an electron transfer mediator, and the step (iii) and the step (v) to provide.