首页> 外国专利> METHOD FOR PRODUCING HIGH CONCENTRATION CADAVERINE BY IMMOBILIZING DECARBOXYLASE-OVEREXPRESSING RECOMBINANT ESCHERICHIA COLI BY USING BARIUM-ALGINATE CARRIER

METHOD FOR PRODUCING HIGH CONCENTRATION CADAVERINE BY IMMOBILIZING DECARBOXYLASE-OVEREXPRESSING RECOMBINANT ESCHERICHIA COLI BY USING BARIUM-ALGINATE CARRIER

机译:海藻酸钡载体固定化过羧羧化酶过表达重组大肠埃希氏菌生产高浓度金刚烷胺的方法

摘要

The present invention relates to recombinant Escherichia coli having introduced lysine decarboxylase derived from Escherichia coli, and to a method for producing an immobilized procell enzyme by using the recombinant protein and producing high concentration cadaverine by using the same, wherein the immobilized procell enzyme is not dissolved even when high concentration cadaverine is produced. According to the present invention, a method for immobilizing barium-alginate is introduced by solving a problem of a conventional method for immobilizing calcium-alginate. When cadaverine is produced from high concentration L-lysine by utilizing the same, high efficiency and stability are ensured, and re-usage problems are overcome. Through a repeatedly utilizable process, economical feasibility can be ensured.;COPYRIGHT KIPO 2017
机译:本发明涉及导入有大肠杆菌衍生的赖氨酸脱羧酶的重组大肠杆菌,以及使用该重组蛋白质生产固定化Procell酶并使用该重组蛋白生产高浓度尸胺的方法,其中固定化的Procell酶不溶解即使产生高浓度的尸胺。根据本发明,通过解决常规的固定海藻酸钙的方法的问题,引入了固定海藻酸钡的方法。当利用尸胺从高浓度的L-赖氨酸生产尸胺时,确保了高效率和稳定性,并且克服了再利用问题。通过反复使用的过程,可以确保经济可行性。; COPYRIGHT KIPO 2017

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