Method for non-specifically extending base sequences of target polynucleotide primer composition for performing the method and kit for improving sensitivity of detecting target polynucleotide

摘要

In the present invention, it is an embodiment thereof that a target polynucleotide that nonspecifically extends a nucleotide sequence includes: (a) a first primer that specifically binds to a target polynucleotide; a genomic gene at a terminus thereof; Preparing a modified first primer having a first non-homologous base sequence or a complementary sequence complementary to the genomic gene as a sequence, (b) not hybridizing with the target polynucleotide complementarily, a method of preparing an extended polynucleotide for which is used to extend the road, the target polynucleotide to non-specific, (c) it is the second primer which genomic gene at the terminal to combine the road extended polynucleotide and specific for homology with the nucleotide sequence The nucleotide sequence which does not have the nucleotide sequence (A) a first non-homologous base sequence which is complementary to the first non-homologous base sequence, (b) a second non-homologous base sequence complementary to the first non-homologous base sequence, or road by using a modification of the first primer to obtain a road first emergency Same nucleotide sequence or its complementary nucleotide sequence a road target polynucleotide is added to the terminus of the target polynucleotide extension, and using the roads modification of the second primer second emergency Same nucleotide sequence or in a step of complementary base sequences road give the extended polynucleotide for a more extended polynucleotide terminal for extension, (e) the road targets, the road added to the polynucleotide-terminal first emergency Same nucleotide sequence or A complementary base sequence and a polynucleotide The first addition to the de-terminal includes the step of extending for a second emergency Same nucleotide sequence or its complementary sequence of the target polynucleotide and the extended polynucleotide for obtained in the road (d) step to the annealing so that the alternating complementary to each other to each other.;