Transactive response DNA binding protein-43TDP-43-GAL4 TDP-43-GAL4 UAS-Green fluorescent proteinGFP TDP-43-GAL4 Drosophila mutants and TDP-43-GAL4 and UAS-GFP Drosophila double mutants and manufacturing method of the same

摘要

The present invention relates to a transactivation response (TAR) DNA binding protein-43 (TDP-43) -GAL4 Drosophila mutant, a TDP-43-GAL4 and UAS-Green fluorescent protein (GFP) Drosophila double mutant, More specifically, it is possible to express fluorescent proteins in tissues in which Drosophila TDP-43 gene is expressed. TDP-43-GAL4 and Green fluorescent protein (GFP) which can confirm the expression position and expression pattern of Drosophila TDP- ) Overexpression mutant Drosophila models and methods for their manufacture. According to the present invention, a tissue-specific expression of TDP-43 gene produced by separating the promoter region of TDP-43 gene from Drosophila genome by using GAL4 system, which is an expression system capable of overexpressing gene transcription in a specific tissue Wherein the Drosophila dual mutants are used for the screening of TDP-43-related factors as well as the function of the TDP-43 gene of Drosophila and the degeneracy of human It can be used as an animal model which is very useful for studying brain disease gene function.