A METHOD FOR ANALYZING BASE SEQUENCE OF GENE TARGET REGION AND A COMPOSTION FOR ANALYZING BASE SEQUENCE OF GENE TARGET REGION

摘要

The present invention relates to a method for analyzing a gene target site base sequence and a composition for analyzing a gene target site sequence using the method, and a method for efficiently and accurately amplifying and analyzing a short target site having a plurality of mutations is. Specifically, by controlling the amplification of a short target site to a restriction enzyme, a polymerase and a reaction temperature, the starting point of synthesis is the same, and various stepwise amplification products are obtained at the termination point. By analyzing the mass of this product, it is possible to perform sequencing of short target sites.