TALEN Transcription Activation Like Effector Nuclease for Targeting Myostatin Gene and Methods for Making Myostatin Gene Knock out Animal Using Thereof

摘要

The present invention relates to a transcription activator like effector-nuclease (TALEN) targeting a myostatin (MSTN) gene and a method for producing an animal in which a myostatin gene is knocked out using the same. In the present invention, it was confirmed that when TALEN and somatic cell nuclear transfer technology specific to myostatin gene are used, transgenic pigs can be effectively knocked out with myostatin gene. Therefore, by using the method of producing a transgenic animal in which the myostatin gene of the present invention is knocked out, it is possible to effectively produce pigs with increased muscle mass, and this method can be used in other animals supplying meat to humans.