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Single nucleotide polymorphism probe for high-throughput genotype analysis of Brassica rapa and use thereof

机译:用于甘蓝型油菜高通量基因型分析的单核苷酸多态性探针及其应用

摘要

The present invention relates to a single nucleotide polymorphism (SNP) probe for use in the analysis of large genotypes of Chinese cabbage, and more particularly, to a method for detecting a single nucleotide polymorphism selected from among 194 single nucleotide polymorphisms, A kit, a microarray DNA chip, and a method for identifying a system or a variety of Chinese cabbage using the probe, the composition, the kit, and the microarray DNA chip.According to the present invention, it is possible to quickly, accurately, and easily discriminate a system or a variety of Chinese cabbages. In particular, since the present invention utilizes the genetic information of Chinese cabbage, it is possible to use a variety of Chinese cabbage tissues or cells such as seeds, sprouts, roots, and the like to determine strains or cultivars even if they are not adults. Therefore, it can be very useful for the development of new varieties of Chinese cabbage, and it is expected that the time required for the development of varieties can be greatly shortened.
机译:本发明涉及用于分析大白菜大基因型的单核苷酸多态性(SNP)探针,更具体地,涉及一种检测选自194种单核苷酸多态性的单核苷酸多态性的方法。微阵列DNA芯片,以及使用该探针,组合物,试剂盒和微阵列DNA芯片鉴定系统或各种大白菜的方法。根据本发明,可以快速,准确和容易地区分系统或各种大白菜。特别地,由于本发明利用了大白菜的遗传信息,因此即使它们不是成年的,也可以使用各种大白菜组织或细胞例如种子,芽,根等来确定菌株或品种。 。因此,它对于大白菜的新品种的开发非常有用,并且有望大大缩短品种开发所需的时间。

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