Process for differentiation of cardiovascular cells from induced pluripotent stem cells prepared by human circulation multipotent adult stem cellsCiMS and uses thereof

摘要

The present invention relates to a method for producing pluripotent pluripotent stem cells derived from endocardial derived adult stem cells isolated from peripheral blood, a method for differentiating into pluripotent stem cells from the pluripotent pluripotent stem cells prepared by the method, and uses thereof.The intracardiac-derived adult stem cells, which are the primary cultured cells for the production of the induced pluripotent stem cells of the present invention, can be easily isolated and cultured even with a small amount of peripheral blood, can be stored without genetic mutation since they have high proliferation power, The number of cells that can be used can be quickly secured. In addition, since stem cells have stem cells, the production efficiency of induced pluripotent stem cells through the transduction of 4 kinds of Yamanaka factor is higher than that of conventional skin fibroblasts, and since they have endocardial origin, Since it has memory, induction pluripotent stem cells can be produced and then differentiated into cardiovascular cells such as endothelial cells, smooth muscle cells and myocardial cells with high efficiency. Therefore, the cardiovascular cells differentiated from the induced pluripotent stem cells can be used for library screening of a therapeutic substance for the development of a specific cardiovascular disease drug, and for studying the mechanism of cardiovascular diseases, It is expected to be widely used in various fields such as the use as a cell therapy agent by injecting again.