Domain CNAB SDRE STAPHYLOCOCCUS AUREUS and its use for vaccination

摘要

1. A polypeptide containing the CnaBE3 SdrE domain, where the polypeptide does not contain (1) a full-sized SdrE protein or (2) the amino acid sequence of formula 'B'.2. A polypeptide comprising a S.aureus SdrE protein fragment, wherein (a) the fragment comprises a CnaBE3 SdrE domain; (b) the polypeptide does not contain a full-sized SdrE protein; and (c) the polypeptide does not contain the amino acid sequence of formula 'B'.3. The polypeptide of claim 2, wherein the SdrE protein is 90% or more identical to SEQ ID NO: 3. The polypeptide according to any one of paragraphs. 1-3, where the CnaBE3 domain is at least 95% identical to SEQ ID NO: 8.5. The polypeptide according to any one of paragraphs. 1-3, containing SEQ ID NO: 8 or SEQ ID NO: 27.6. The polypeptide according to any one of paragraphs. 1-3, which, when administered to a human or mouse, causes the production of antibodies that recognize the epitope in SEQ ID NO: 8 or in SEQ ID NO: 27.7. The polypeptide according to any one of paragraphs. 1-3, having less than 500 amino acids. 8. A polypeptide containing a mutant domain of CnaBE3 SdrE S.aureus, where: - in one or more than one position of the amino acid, where the native domain of CnaBE3 SdrE S.aureus has an asparagine residue, the mutant has either (1) an amino acid deletion, or (2) amino acid substitution ; and / or - in one or more than one position of the amino acid, where the native CnaBE3 SdrE domain of S.aureus has an aspartate residue, the mutant has either (1) an amino acid deletion, or (2) amino acid substitution; and / or - in one or more than one amino acid position, where the native CnaBE3 SdrE domain of S.aureus has a lysine residue, does the mutant of (1) a deletion of amino acids, or (2) replace aminokisloty.9. The polypeptide according to claim 8, containing any of SEQ ID NO: 9-26.10. A polypeptide containing the S. aureus CnaB domain, wherein the CnaB domain includes an isopeptide bond. 11. The polypeptide of claim 10, wherein the S. aureus CnaB domain is derived from S. aureus SdrE. 12. The polypeptide of claim 11, wherein the S. aureus CnaB domain is a CnaBE3.13 domain. A polypeptide containing at least two CnaB domains, where: either (a) at least one of the CnaB domains is a CnaBE3 SdrE domain and at least one CnaB domain is not a CnaB SdrE domain; or (b) a polypeptide