METHOD certain drugs and their metabolites in human blood

摘要

A method for determining psychoactive substances and their metabolites in human blood under chemical-toxicological analysis comprising deposition of protein and lipid analysis sample, extraction of the desired substances, separation of the layers and subsequent GC-MS extract analysis and detection of the mass spectra in the full scan mode, wherein the deposition of proteins and lipids, the extraction of the desired substances and separation of the layers is performed simultaneously and directly into the vial, the vial successively placed in the calculated amount of crystalline sodium chloride solution, poured estimated quantities of the internal standard, the samples analyzed blood and acetonitrile, then the mixture thus obtained system was homogenized and extracted by stirring at vibromiksere, the layers were separated by centrifugation at 15,000 rpm / min for analyzing collected extract needle autosampler from the bottom of the upper layer volume without contact with the boundary between the layers, and the presence of PA is determined by comparing the mass spectra recorded with a library of reference data analytical characteristics of the desired substances.