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METHOD FOR DIAGNOSTIC AVAILABILITY OF MUTATION 1727T C OF THE KIT GENE IN MELANOMA TISSUES
METHOD FOR DIAGNOSTIC AVAILABILITY OF MUTATION 1727T C OF THE KIT GENE IN MELANOMA TISSUES
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机译:黑色素瘤组织中突变基因1727T> C的诊断可用度的方法
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摘要
FIELD: biotechnology.SUBSTANCE: invention is intended to diagnose the presence of c-KIT gene L576P mutation in melanoma tissues. Estimation of the presence of mutation is carried out according to the ratios of the values of Ct threshold cycles in three real-time PCR reactions with allele-specific primers. Based on the results of three duplicate reactions, the mutant DNA proportion in the sample is calculated.EFFECT: invention provides optimisation of real-time allele-specific PCR for diagnosis of c-KIT L576P tyrosine kinase receptor mutation in melanoma tissues.6 dwg, 1 tbl, 4 ex
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机译:技术领域:本发明旨在诊断黑素瘤组织中c-KIT基因L576P突变的存在。根据等位基因特异性引物的三个实时PCR反应中Ct阈值循环值的比率,对突变的存在进行估计。基于三个重复反应的结果,计算样品中突变DNA的比例。效果:本发明提供了实时等位基因特异性PCR的优化,用于诊断黑素瘤组织中的c-KIT L576P酪氨酸激酶受体突变。6dwg, 1汤匙,4 ex
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