METHOD OF PRODUCING WATER-SOLUBLE POLYSACCHARIDES OF COMMON BURDOCK ROOTS

摘要

FIELD: chemistry.;SUBSTANCE: method comprises exhaustive extraction of starting material with water, separation of plant material, deposition of water-soluble polysaccharides, washing and drying. Starting material used is common burdock roots ground to linear size of 0.2-0.5 mm . Extraction is three-fold with hot purified water in ratio of raw material and extractant of 1:30 when processing in ultrasonic bath with frequency 35 kHz for 30 minutes at a temperature of 80 °C. Plant material is separated by filtration, and water-soluble polysaccharides are deposited three-fold amount of 95 % ethanol while stirring, cooling in freezing chamber at temperature -18 °C for 1 hour. Precipitate is filtered through a dry ash-free paper filter under vacuum at residual pressure of 0.4-0.8 atm. Filter cake is washed successively with a solution of 95 % ethyl alcohol in purified water (3:1) with ratio of raw material and solution of 1:15 and mixture of ethyl acetate and 95% ethyl alcohol (1:1) at a ratio of raw material and solution of 1:10. Filter with precipitate is then dried at a temperature of 100-105 °C to constant weight.;EFFECT: invention reduces duration of extraction and increases output of water-soluble polysaccharides.;1 cl, 3 dwg, 2 ex