EXPRESSION SYSTEM AND METHOD OF PRODUCING NON-MODIFIED RECOMBINANT PROTEINS IN Escherichia coli USING IT

摘要

FIELD: biotechnology.;SUBSTANCE: group of inventions relates to biotechnology. DNA construct is disclosed, which codes fused precursor protein, in which auxiliary amino acid sequence is bound to N-end of mature target polypeptide sequence with transition site intended for identification and splitting of hybrid precursor by specific protease to form non-modified protein of interest. Above site represents truncated at N-end by 23 amino acid residues SUMO protein of S. cerevisiae (Smt3), which includes site of splitting with SUMO-hydrolase (Ulp1). On basis of DNA construct coding version of precursor, which comprises glutathione-S-transferase (GST) as auxiliary sequence and negatively charged peptide and human interferon (IFN) α2b as target protein recombinant E. coli strain is obtained. Method of producing non-modified IFN α2b using above E. coli strain is also disclosed.;EFFECT: group of inventions enables to obtain target protein with high purity and activity.;7 cl, 9 dwg, 2 tbl, 5 ex