METHOD OF FORECASTING PERSONSIVE SURVIVABILITY IN PATIENTS WITH BREAST CANCER

摘要

FIELD: medicine.;SUBSTANCE: determining the level of expression of YKL-39 using TaqMan technology using specific primers and Sense probe 5'-aacaacaaggttatcatcaaggac-3', AntiSense 5'-tttgggattcttggttttgag-3', Probe FAM-5'-agtgaagtgatgctctaccagaccat-3'-BHQ1, PCR analysis of the expression of the YKL-39 protein gene, the relative expression of the YKL-39 protein gene was evaluated using the Pfaffl method in conventional units (CU) relative to the GAPDH referee gene. As the calibrator, normal breast tissue is used, in which, in the same manner as described above, the level of expression of the YKL-39 protein gene is 1 CU. With an expression level of YKL-39, more than 1 CU is predicted to be high, and at a YKL-39 protein expression level less than 1 CU, a low probability of a prolonged period of non-metastatic survival.;EFFECT: higher prediction accuracy.;1 tbl