RECOMBINANT L1HPV16 GENE, RECOMBINANT PQE-L1/16 PLASMID, L1HPV16 PROTEIN AND THEIR APPLICATION

摘要

FIELD: biotechnology.;SUBSTANCE: recombinant gene encoding a human papillomavirus 16 type L1 protein is provided, with codons optimised for heterologous expression in E. coli strains. An expression plasmid vector pQE-L1/16 carrying this recombinant gene is presented. A method for recombinant L1HPV16 protein production using this vector, which is used to transform E. coli strains is provided. An immunogenic composition and vaccine are provided for induction of specific cellular and humoral immunity against HPV16 containing recombinant L1HPV16 protein.;EFFECT: group of inventions allows to obtain a stable purified immunogenic L1HPV16 protein, allows to reduce the cost of its preparation process for use in effective immunogenic compositions and vaccines against type 16 human papillomavirus.;6 cl, 3 dwg, 1 tbl, 5 ex