Confirmatory test for primary nucleic acid amplificates in a continuous reaction mixture and immediate evaluation by means of immunochromatographic methods

摘要

The present invention relates to a method for confirming amplified nucleic acid target sequence (target sequence), preferably from human samples, during a amplification reaction in a collective and continuous reaction batch as a one-pot process wherein the confirmation of the target sequence amplicon is obtained via a hapten-pair-labeled template artificial amplificate. In this case, the artificial template sequence is amplified by means of the 5'-cleavage products of the at least one target sequence-specific FEN probe and optionally labeled. The 5 'cleavage product of the at least one target sequence-specific FEN probe is obtained only when the FEN probe hybridizes with its target sequence-specific 3' sequence to a complementary sequence portion of the at least one target sequence. The detection of the obtained plurality of template amplificates takes place distinctly and preferably by means of immunochromatographic methods.