Brief description of the transcriptional amplification of nucleic acids bringing together of the various steps of temperatures

摘要

The present invention relates to a method of the transcriptional amplification, in which: a) at least one target nucleic acid present in a biological sample, is placed in contact: amplification primers,.. of the reagents necessary for the production of the amplification, the enzymes involved in the amplification, and of at least one polyol, which makes it possible to stabilize the enzymes that are necessary for the production of the amplification, b) the mixture is heated at a temperature higher than 41°C, c) it provides an amplification of the transcriptional target nucleic acid at a temperature higher than 41°C. the invention also relates to a process for the detection of amplicons obtained by the amplification method, a process for the pretreatment of the target nucleic acid sought, and a process for the in vitro diagnosis of the presence of the target nucleic acid or of these. It finds a preferred application in the field of medical diagnostic.