COMPOSITION FOR HOT-START REVERSE TRANSCRIPTION REACTION OR HOT-START REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION

摘要

The present invention relates to a composition for hot-start reverse transcription reaction and a composition for reverse transcription PCR, which comprises the same, and more particularly to a reaction composition obtained by adding pyrophosphate and pyrophosphatase to an aqueous solution containing reaction buffer solution, MgCl 2 , four kinds of dNTPs, and reverse transcription polymerase in a single reaction tube, and a composition for hot-start reverse transcription reaction obtained by freezing or drying the reaction composition and having increased stability and long- term storage stability. Also, the present invention relates to a composition that comprises DNA polymerase in the composition for hot-start reverse transcription reaction, and thus enables a hot-start reverse transcription reaction and a PCR reaction to be sequentially performed, and to a method for amplifying nucleic acid the same. In comparison with conventional compositions for reverse transcription reaction, the composition for hot-start reverse transcription reaction can prevent non-specific reverse transcription reactions during mixing at room temperature and can selectively perform the reverse transcription of the target RNA at the reaction temperature, making it possible to amplify the reverse transcription product by PCR with high sensitivity. Thus, the composition of the invention can be conveniently and effectively used in multiplex reverse transcription PCRs or real-time quantitative reverse transcription PCR.