首页>
外国专利>
ASSAY FOR MONITORING PORCINE CIRCOVIRUS PRODUCTION
ASSAY FOR MONITORING PORCINE CIRCOVIRUS PRODUCTION
展开▼
机译:监测猪圆环病毒生产的检测
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention provides methods for the determination of the viral titer of a culture of host animal host cells infected with a circovirus. The FACS-based methods of the invention may include determining the viability of the host cells in a cell culture medium supernatant and of those cells that remain adherent to a solid support. Detecting and measuring the percentage of cells that expressed the viral antigens ORF1 and OFR2 may determine the viral load of the cultured host cells. The yield of the virus may be established by the detection and measurement of both antigens in supernatant cells, for example (5) to (7) days from when the host cells are transferred to a serum free medium. The methods of the invention may yield rapid quantitative data. This allows the repeated in-process monitoring of the viral production throughout the incubation period, and ready selection of the most appropriate harvesting point.
展开▼