首页>
外国专利>
MEASUREMENT OF PROTEIN MOLECULAR FLUX RATES BY QUANTIFYING ISOTOPOLOGUE ABUNDANCES IN IMMONIUM ION FRAGMENTS USING HIGH RESOLUTION MASS SPECTROMETRY
MEASUREMENT OF PROTEIN MOLECULAR FLUX RATES BY QUANTIFYING ISOTOPOLOGUE ABUNDANCES IN IMMONIUM ION FRAGMENTS USING HIGH RESOLUTION MASS SPECTROMETRY
展开▼
机译:高分辨率质谱法定量离子态离子片段中的同位素异构体丰度,从而测量蛋白质的分子通量
展开▼
页面导航
摘要
著录项
相似文献
摘要
Provided herein are methods for measuring a molecular flux rate proteins and polypeptides based on analysis of isotopologue abundances of immonium ion fragments within a mass isotopomer using a high resolution mass spectrometric measurement. Such methods may be used, inter alia, to calculate a fraction of newly synthesized protein or polypeptide molecules of interest, a replacement rate of target molecules of interest, and/or a rate of breakdown or degradation of target molecules of interest, e.g., based on isotopologue relative abundances.
展开▼