METHOD FOR BUILDING DNA LIBRARY FOR SEQUENCING

摘要

Provided is a method for constructing a DNA library for sequencing. The method comprises A-tailing of a single 3' end to at least a portion of blunt-end DNA fragments, and a step of blunt-end ligating the obtained DNA fragments having A tailing to the single 3' end. Also provided are a DNA library for sequencing constructed with the method and a corresponding sequencing method.