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CELL CULTURE METHOD, TARGET CELLS CULTURED BY CELL CULTURE METHOD, AND CELL CULTURE DEVICE

机译:细胞培养方法,通过细胞培养方法培养的靶细胞和细胞培养装置

摘要

PROBLEM TO BE SOLVED: To suppress adverse effects caused by the heat of a laser beam on target cells when removing unwanted cells by a laser beam.SOLUTION: A cell culture method for culturing target cells while removing unwanted cells when target cells to be cultured and cells other than the target cells which are unwanted are present in a culture solution in a culture vessel comprises: a step of laser beam irradiation which irradiates unwanted cells with a laser beam in order to remove the unwanted cells which have adhered to the culture vessel; a culturing step of culturing the target cells which is carried out after laser beam irradiation step; and a step of culture solution exchange in which the culture solution including the unwanted cells removed from the culture vessel is exchanged for new culture solution which is carried out after the culturing step.SELECTED DRAWING: Figure 6
机译:解决的问题:当用激光束去除不需要的细胞时,抑制激光束的热量对靶细胞产生的不利影响解决方案:一种细胞培养方法,用于培养靶细胞,同时去除待培养的靶细胞时去除不需要的细胞存在于培养容器的培养液中的除了靶细胞以外的不需要的细胞包括:激光束照射步骤,用激光束照射不需要的细胞,以除去附着在培养容器上的不需要的细胞。在激光束照射步骤之后进行的培养靶细胞的培养步骤;以及培养液交换步骤,其中将包括从培养容器中移出的多余细胞的培养液交换为在培养步骤之后进行的新的培养液。图6

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