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Method of examining a plurality of cultured cells for the presence of a periodic structure of at least one target component contained in the cultured cells

机译:检查多个培养细胞中是否存在培养细胞中至少一种靶成分的周期性结构的方法

摘要

A method for examining cultured cells for the presence of periodic structures comprises the steps of: - providing cultured cells, - fixing the cultured cells, - staining the fixed cultured cells using a first staining agent, - stimulating the first staining agent causing it to emit light, - taking a two-dimensional image (2) of the fixed cultured cells, - cross-correlating first and second filtered images derived from the image (2) of the fixed cultured cells to obtain a correlation image (3), and - determining the presence or absence of periodic structures in the fixed cultured cells by determining whether there are periodic structures of maxima and minima in brightness in the correlation image (3).
机译:一种检查培养细胞中是否存在周期性结构的方法,包括以下步骤:-提供培养的细胞,-固定培养的细胞,-使用第一染色剂对固定的培养细胞进行染色,-刺激第一种染色剂使其发光,-拍摄固定培养细胞的二维图像(2),-从固定培养细胞的图像(2)得到的第一和第二滤波图像互相关以获得相关图像(3),和-通过确定相关图像中亮度中是否存在最大值和最小值的周期性结构来确定固定培养细胞中是否存在周期性结构(3)。

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