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CELL CULTURE METHOD, TARGET CELLS CULTURED BY SAID CELL CULTURE METHOD, AND CELL CULTURE DEVICE

机译:细胞培养方法,通过所述细胞培养方法培养的靶细胞和细胞培养装置

摘要

The present invention curbs the adverse effects of heat from laser light on target cells when unwanted cells are being stripped away by the laser light. A method for culturing target cells while also removing unwanted cells in a case where cells present in a culture medium placed in a culture vessel include the target cells for which the culture is intended, and the unwanted cells which are cells other than the target cells, wherein the method comprises: a laser light irradiation step for irradiating unwanted cells adhering to a culture vessel with laser light in order to strip the unwanted cells away from the culture vessel; a culture step, executed following the laser light irradiation step, for culturing the target cells; and a culture medium replacement step, executed following the culture step, for replacing the culture medium that includes the unwanted cells that have been stripped away from the culture vessel with a fresh one.
机译:当不需要的细胞被激光剥离时,本发明抑制了来自激光的热量对靶细胞的不利影响。在放置于培养容器中的培养基中存在的细胞包括要进行培养的靶细胞以及除靶细胞以外的细胞以外的不需要细胞的情况下,用于在去除靶细胞的同时进行靶细胞的培养的方法,其中,该方法包括:激光照射步骤,用激光照射粘附到培养容器上的不需要的细胞,以便从培养容器上剥离掉不需要的细胞。在激光照射步骤之后执行的培养步骤,用于培养靶细胞;在培养步骤之后执行的培养基替换步骤,用于用新鲜的培养基替换包括从培养容器中剥离下来的不需要细胞的培养基。

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