METHOD FOR SEPARATING ANTIBODIES HAVING DIFFERING COMPLEMENT DEPENDENT CYTOTOXIC ACTIVITIES

摘要

PROBLEM TO BE SOLVED: To provide a method for separating antibodies having differing complement dependent cytotoxic activities.;SOLUTION: A sample containing antibodies having differing complement dependent cytotoxic activities is made to contact an adsorbent obtained by immobilizing an Fc binding protein (preferably human FcγRIIIa, more preferably human FcγRIIIa not having a sugar chain) on an insoluble carrier. The antibodies are adsorbed onto the adsorbent. Thereafter, antibodies with differing CDC activities are sequentially eluted by weakening the interaction between the antibodies and Fc binding protein, and antibodies with different complement dependent cytotoxic activities are separated.;SELECTED DRAWING: Figure 3;COPYRIGHT: (C)2018,JPO&INPIT