PRODUCTION OF HETEROLOGOUS POLYPEPTIDES IN MICROALGAE, MICROALGAL EXTRACELLULAR BODIES AND COMPOSITIONS, AND METHODS OF MAKING AND USES THEREOF

摘要

PROBLEM TO BE SOLVED: To provide a method for producing viral hemagglutinin (HA) protein using microalgae.SOLUTION: Provided is a method for producing a full length influenza HA protein as a vaccine using a recombinant microalgal cell, where the microalgal cell comprises a nucleic acid molecule comprising a polynucleotide sequence encoding a viral HA protein including a HA membrane domain as well as active regulatory control elements, the microalgal cell is a Schizochytrium or a Thraustochytrid, and the recombinant microalgal cell is cultured in a culture medium into which the HA protein is secreted. Also provided is a method for formulating the recombinant viral protein as a vaccine composition, where the protein is an influenza protein.SELECTED DRAWING: None