Highly sensitive biomarker quantification method using photooxidation amplification

摘要

A highly sensitive biomarker quantification method using photooxidation amplification is disclosed. A high-sensitivity biomarker quantification method using photo-oxidation amplification according to an embodiment of the present invention includes an enzyme labeled with any one selected from an antibody, an aptamer, and a nucleic acid that specifically bind to a biomarker. A step of performing an enzyme-substrate reaction on the measurement sample, and after the enzyme-substrate reaction, a photo-oxidation amplification process is performed by continuously exposing light to the product, and a color development amount of the product during the photo-oxidation amplification process Optically measuring at least one light characteristic selected from the amount of luminescence and the amount of fluorescence; indexing a change aspect with time for the measured optical characteristic; and the indexing process And comparing the index extracted in step 1 with the index of the reference sample to quantify the concentration of the biomarker contained in the measurement sample. Therefore, after the reaction between the enzyme and the substrate labeled with the antibody, aptamer, or nucleic acid specific to the biomarker to be detected and the substrate, the product is exposed to light continuously to perform the photo-oxidative amplification process. As a result, the low-level or trace amount of biomarker can be quantified more accurately and with high sensitivity.