Suppression of errors in sequencing DNA fragments using redundant reads with specific molecular index (UMI)

摘要

The disclosed embodiments are for determining a sequence of interest using a specific molecular index (UMI) sequence that is specifically associated with an individual polynucleotide fragment, including sequences with low allele frequency and long sequence length. The present invention relates to methods, apparatus, systems, and computer program products. In some embodiments, the UMI comprises both a physical (exogenous) UMI, eg, introduced using a Y-type adapter, and a virtual (endogenous) UMI present in the DNA fragment being sequenced. Including. In some embodiments, the specific molecular index sequence comprises a non-random sequence. Systems, apparatus, and computer program products for performing the disclosed methods to determine a sequence of interest are also provided. [Selection] Figure 3B