5-Hydroxymethylcytosine oxidizing agent and 5-hydroxymethylcytosine analysis method

摘要

Inexpensive and novel 5-hydroxymethylcytosine oxidant that suppresses DNA structure destabilization and side reactions and selectively oxidizes 5-hydroxymethylcytosine on DNA to convert it to 5-formylcytosine And a method for detecting a demethylation site of DNA demethylation with high accuracy using the oxidizing agent. Provided is a 5-hydroxymethylcytosine oxidizing agent comprising a nitroxyl radical molecule and a copper salt or a copper complex, and / or a nitroxyl radical molecule-copper complex.