Systems and methods of dissociating aggregate spheres of cells

摘要

Various implementations include systems and methods for automatically and continuously dissociating spheres of cells. In particular, one such system includes a peristaltic pump in fluid communication via tubing with a bioreactor in which cells are cultivated. A plurality of conduits are disposed within a portion of the tubing such that fluid flowing between the bioreactor and the pump passes through the conduits. The conduits have an inner diameter that is sized to provide a shear stress to the fluid of between about 5 to about 60 dynes/cm², which is sufficient for dissociating the spheres of cells passing through the conduits. For example, each conduit may have an inner diameter of between about 50 and about 250 micrometers.