The object of the present invention is to provide a bacterial flora analysis method for quantitatively evaluating bacterial flora and to provide a device used in the method. The device relating to the present invention is a device mounted with (a) a probe consisting of a nucleic acid hybridizing to 16SrRNA specific to each of 1 kind or 2 or more kinds of bacteria to be a subject of detection, and at least one of (b) a total amount indicator probe and (c) one or more kinds of an absolute amount indicator probe.
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