首页>
外国专利>
DYNAMIC MONITORING OF RECEPTOR TYROSINE KINASE (RTK) ACTIVITY AND PATHWAYS IN LIVING CELLS USING REAL-TIME MICROELECTRONIC CELL SENSING TECHNOLOGY
DYNAMIC MONITORING OF RECEPTOR TYROSINE KINASE (RTK) ACTIVITY AND PATHWAYS IN LIVING CELLS USING REAL-TIME MICROELECTRONIC CELL SENSING TECHNOLOGY
展开▼
机译:利用实时微电子细胞传感技术动态监测生活细胞中的受体酪氨酸激酶(RTK)活性和途径
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method of identifying a potential therapeutic compound that affects a Receptor Tyrosine Kinase (RTK) pathway in cancer cells, which includes: providing a device capable of measuring cell-substrate impedance; culturing cancer cells in serum-free media in at least two wells of the device; adding to a first well a proposed therapeutic compound that affects a RTK pathway and a RTK stimulating factor for the RTK pathway to form a test well, and adding to another well the RTK stimulating factor to form a control well; continuously monitoring cell-substrate impedance of the at least two wells and optionally determining cell indices from the monitored cell-substrate impedance; and determining a difference in impedance or optionally cell index between the test well and control well; and if significantly different, concluding the proposed therapeutic compound is therapeutically active in the RTK pathway within the cancer cells.
展开▼