METHOD FOR DETECTING PRODUCTS DERIVED FROM GLUCURONIDE METABOLITES WITH THE ENZYME B-GLUCURONIDASE, AND A REAGENT COMPRISING SAID ENZYME

摘要

The present invention discloses a method and a highly efficient reagent to detect products derived from glucuronide metabolites presents in a sample, comprising the steps of adding to said sample an enzyme with β-glucuronidase activity originated from genus Brachyspira or any variant or mutant derived thereof; incubating the sample with the enzyme during a determined period of time; and detecting the product derived from glucuronide metabolite by means of a suitable technique. The reagent of the present invention comprises an enzyme with β-glucuronidase activity originated from genus Brachyspira or any variant or mutant derived thereof; and a suitable vehicle.