首页> 外国专利> Digital microscope comprising pivoting stand, method for calibration and method for automatic focus and image center tracking for such a digital microscope

Digital microscope comprising pivoting stand, method for calibration and method for automatic focus and image center tracking for such a digital microscope

机译：包括枢转支架的数字显微镜，用于该数字显微镜的校准方法以及自动聚焦和图像中心跟踪的方法

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摘要

A digital microscope having a pivoting stand, a method for calibrating said stand and a method for automatic focus tracking and image center tracking upon actuation of the pivoting stand. The pivoting stand includes an angle sensor for determining a current pivot angle of the pivot arm (07). The current pivot angle is processed in the control unit to execute automatic focus tracking and/or center tracking upon actuation of the pivot arm (07). Calibration is performed using two pivot angles, wherein deviating focus and image center positions are ascertained, and a pivot-angle-dependent function for focus and the image center position is ascertained therefrom.

机译：具有枢轴支架的数字显微镜，用于校准所述支架的方法以及在枢轴支架致动时用于自动聚焦跟踪和图像中心跟踪的方法。枢转台包括用于确定枢转臂的当前枢转角的角度传感器（ 07 ）。当前枢轴角度在控制单元中进行处理，以便在枢轴臂（ 07 ）致动时执行自动聚焦跟踪和/或中心跟踪。使用两个枢轴角执行校准，其中确定偏离的焦点和图像中心位置，并由此确定焦点和图像中心位置的依赖于枢轴角度的功能。 展开▼

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公开/公告号US9817223B2

专利类型

公开/公告日2017-11-14

原文格式PDF

申请/专利权人 CARL ZEISS MICROSCOPY GMBH;
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申请/专利号US201415021809

发明设计人 DETLEF HEIN;
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申请日2014-10-28

分类号G02B21/00;G02B21/24;G02B21/26;F16M11/04;F16M11/18;F16M11/20;G02B21/36;

国家 US

入库时间 2022-08-21 12:57:05

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1. 包括枢转支架的数码显微镜，该数码显微镜的校准方法和自动对焦及图像中心跟踪的方法 [P] . 中国专利： CN105683802B . 2018.06.22

2. 包括枢转支架的数码显微镜，该数码显微镜的校准方法和自动对焦及图像中心跟踪的方法 [P] . 中国专利： CN105683802A . 2016-06-15

3. DIGITAL MICROSCOPE COMPRISING PIVOTING STAND, METHOD FOR CALIBRATION AND METHOD FOR AUTOMATIC FOCUS AND IMAGE CENTER TRACKING FOR SUCH A DIGITAL MICROSCOPE [P] . 美国专利： US2016231552A1 . 2016-08-11

机译：包括支架的数字显微镜，标定方法以及这种数字显微镜的自动焦点和图像中心跟踪方法

4. DIGITAL MICROSCOPE COMPRISING PIVOTING STAND, METHOD FOR CALIBRATION AND METHOD FOR AUTOMATIC FOCUS AND IMAGE CENTRE TRACKING FOR SUCH A DIGITAL MICROSCOPE [P] . 欧洲知识产权局专利： EP3066510A1 . 2016-09-14

机译：包括支架的数字显微镜，标定方法以及这种数字显微镜的自动焦点和图像中心跟踪方法

5. DIGITAL MICROSCOPE COMPRISING PIVOTING STAND, METHOD FOR CALIBRATION AND METHOD FOR AUTOMATIC FOCUS AND IMAGE CENTRE TRACKING FOR SUCH A DIGITAL MICROSCOPE [P] . 世界知识产权组织专利： WO2015063047A1 . 2015-05-07

机译：包括支架的数字显微镜，标定方法以及这种数字显微镜的自动焦点和图像中心跟踪方法

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1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004）（P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期

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