Structural limit value biosynthesis, catalytic benefit reaction to produce three benefits of acrylic acid, food, nutrient or oil, hydrogenation method for producing 1.3-dichloroacrylic acid (DAG) and oil or fat

摘要

The biological activity synthesis method of structured fat includes the following steps: (a) providing a polypeptide with hydrolytic activity, in which the polypeptide is a separated, synthesized or re synthesized polypeptide with hydrolytic activity and the polypeptide in the following order: As described in SEQ ID No. 2, but also modified d61a, d61e, r72e, r72k, e116a, e116q, e116r, e116t, e116v, s33a, i151g, i151a, v163r, or d164r residues,O (II) a series of amino acids, as specified in SEQ ID 2, but also the change of amino acid residue i20l; v62s; g77p; v83c; d88h; y113g; e116t; e116g, h140k; k146s; i167s; L180E; e194m; a211q; s122y; g215c; g215v; g215w; a218h; a218s; v223a; 25a2m; or 25a2q; (b) Provide three triglyceride (tag) components;(c) In this condition, a residue of the hydrogenated peptide, anhydrides, is in the position of cyanuric acid (tag) S2,1. Production of 1,3-silicon dioxide (DAG);(d) R1 ester is provided; (E) a specific R1 hydrolysis is provided; and (f) a 1.3-dag of level (c) is associated with a R1 ester of level (d) and a specific R1 hydrolytic tissue of level (E), in which case the R1 hydrolytic tissue can specifically catalyze the disinfection of S2 position, thereby producing a structured lipid.