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Artificially synthesized a Gene resistant to Insect and its application

机译:人工合成抗虫基因及其应用

摘要

This application discloses an artificially synthesized Gene resistant to Insect and its application.The Insect Resistant Gene provided in this application is obtained by Optimization of the Gene Cry1Ah Gene according to the codon preference of corn and is specifically oneThe following (a) - (c): (a) a DNA Molecule, its Nucleotide sequence is shown as SEQ ID no. 2 in the sequence list; (b) a DNA Molecule.The Nucleotide sequence is shown as SEQ ID position 1 - 2007 no. 3 in the sequence list; and (c) a DNA Molecule.Its Nucleotide sequence has at least 98% identity with SEQ ID no. 3 or 1 - 2007 positions of SEQ ID no. 3 in the list of sequences and encodes the Protein shown by the SEQ ID no. 9.Compared with transgenic Maize introduced with the Gene Cry1Ah before OptimizationThe amount of protein expression Cry1Ah in Transgenic Maize containing the Insect Resistant Gene synthesized artificially provided by the present request Improvement meansGrowth; Meanwhile, The Insect Resistance is also improved significantly.
机译:本申请公开了一种人工合成的抗昆虫基因及其应用。本申请中提供的抗昆虫基因是根据玉米的密码子偏好通过优化Cry1Ah基因获得的,具体为以下(a)-(c)。 :(a)一种DNA分子,其核苷酸序列如SEQ ID No.1所示。序列表中的2; (b)DNA分子。核苷酸序列显示为SEQ ID位置1-2007 no.。序列表中的3;其核苷酸序列与SEQ ID No.1具有至少98%的同一性。 SEQ ID No.3或1-2007位置。序列列表中的3并编码SEQ ID No. 9,与优化前导入基因Cry1Ah的转基因玉米相比,含有本要求人工合成的抗虫基因的转基因玉米中蛋白Cry1Ah的表达量同时,抗虫性也大大提高。

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